Author : Mona El Assal
CoAuthors : Mona El Assal, Mohamed Kassem, Amir Ali, Ali Elbadrawy
Source : International journal of advanced Research
Date of Publication : 11/2017
Abstract :
The overall objective of this research is to improve the oral
bioavailability of insulin through encapsulation in nanoparticles
formulated by "ionotropic pre-gelation followed by polyelectrolyte
complexation technique". The preparation variables such as initial drug
concentration, polymer: polymer ratios, crosslinker concentration,
stirring speed, stirring time, pH of drug / polymer mixture were
investigated to study the effect of variables on nanoparticles size and
drug entrapment efficiency. The optimum formula of insulin loaded
nanoparticles was tested for insulin release in different pH media. The
pharmacological activity of insulin loaded nanoparticles was evaluated
following oral dosage in diabetic rats and then study whether insulin
loaded nanoparticles would induce hypoglycemic effect after oral
administration to diabetic rats. The optimum formula of nanoparticles
improved insulin release characteristics. Thus, the polymer matrix
provided protection for insulin in acidic gastric medium and allowed
prolonged insulin release in alkaline intestinal medium. In vivo results
indicated that nanoparticles kept insulin bioactivity and its
hypoglycemic effect after oral administration of insulin loaded
nanoparticles to diabetic rat model. It was found that natural
biodegradable nanoparticles are a promising device for oral insulin
delivery.
Download PDF