Author : Hayam Lotfy
CoAuthors : Sawsan M. Amer, Hala E. Zaazaa, Noha S. Mostafa
Source : Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Date of Publication : 01/2015
Abstract :
Two novel simple, specific, accurate and precise spectrophotometric methods manipulating ratio spectra
are developed and validated for simultaneous determination of Esomeprazole magnesium trihydrate
(ESO) and Naproxen (NAP) namely; absorbance subtraction and ratio difference. The results were compared
to that of the conventional spectrophotometric methods namely; dual wavelength and isoabsorptive
point coupled with first derivative of ratio spectra and derivative ratio. The suggested methods were
validated in compliance with the ICH guidelines and were successfully applied for determination of ESO
and NAP in their laboratory prepared mixtures and pharmaceutical preparation. No preliminary
separation steps are required for the proposed spectrophotometeric procedures. The statistical comparison
showed that there is no significant difference between the proposed methods and the reported
method with respect to both accuracy and precision
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