Author : Hayam Lotfy
CoAuthors : Sarah S. Saleh, , Nagiba Y. Hassan, Hesham Salem
Source : Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Date of Publication : 01/2015
Abstract :
Novel spectrophotometric methods were applied for the determination of the minor component tetryzoline
HCl (TZH) in its ternary mixture with ofloxacin (OFX) and prednisolone acetate (PA) in the ratio of
(1:5:7.5), and in its binary mixture with sodium cromoglicate (SCG) in the ratio of (1:80). The novel spectrophotometric
methods determined the minor component (TZH) successfully in the two selected mixtures
by computing the geometrical relationship of either standard addition or subtraction. The novel
spectrophotometric methods are: geometrical amplitude modulation (GAM), geometrical induced amplitude
modulation (GIAM), ratio H-point standard addition method (RHPSAM) and compensated area
under the curve (CAUC). The proposed methods were successfully applied for the determination of the
minor component TZH below its concentration range. The methods were validated as per ICH guidelines
where accuracy, repeatability, inter-day precision and robustness were found to be within the acceptable
limits. The results obtained from the proposed methods were statistically compared with official ones
where no significant difference was observed. No difference was observed between the obtained results
when compared to the reported HPLC method, which proved that the developed methods could be
alternative to HPLC techniques in quality control laboratories
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