Author : Hayam Lotfy
CoAuthors : Hesham Salem, Nagiba Y. Hassan and Sarah S. Saleh
Source : Journal of Planar Chromatography
Date of Publication : 01/2015
Abstract :
This work presents a comparative study on the development and
validation of two analytical techniques applied for the simultaneous
determination of hydrocortisone acetate (HCA), fusidic
acid (FSA), methyl paraben (MPB), and propyl paraben (PPB)
formulated as a topical cream. The first technique was thin-layer
chromatography (TLC)–densitometric method, which was developed
by separating the four components on silica gel 60 F254 using
methylene chloride–methanol–benzene in the ratio of 10:2:5, v/v, as
the developing system, followed by densitometric measurement of
the bands at 240 nm. The second technique was the chemometric
method using two models: principle component regression model
(PCR) and partial least squares (PLS). The suggested techniques
were validated in compliance with the International Conference on
Harmonization (ICH) guidelines and were successfully applied for
the determination of the quaternary mixtures as prepared synthetically
in laboratory and in the commercial topical pharmaceutical
formulation.
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