Author : Hayam Lotfy
CoAuthors : Yasmin M. Fayez , Adel M.Michael ,, Christine K. Nessim
Source : Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Date of Publication : 01/2016
Abstract :
Smart, sensitive, simple and accurate spectrophotometric methods were developed and validated for the
quantitative determination of a binary mixture of mebeverine hydrochloride (MVH) and chlordiazepoxide
(CDZ) without prior separation steps via different manipulating pathways. These pathways were applied either
on zero order absorption spectra namely, absorbance subtraction (AS) or based on the recovered zero order absorption
spectra via a decoding technique namely, derivative transformation (DT) or via ratio spectra namely,
ratio subtraction (RS) coupledwith extended ratio subtraction (EXRS), spectrumsubtraction (SS), constant multiplication
(CM) and constant value (CV) methods. The manipulation steps applied on the ratio spectra are namely,
ratio difference (RD) and amplitude modulation (AM) methods or applying a derivative to these ratio spectra
namely, derivative ratio (DD1) or second derivative (D2). Finally, the pathway based on the ratio spectra of derivative
spectra is namely, derivative subtraction (DS). The specificity of the developedmethodswas investigated by
analyzing the laboratory mixtures and was successfully applied for their combined dosage form. The proposed
methods were validated according to ICH guidelines. These methods exhibited linearity in the range of 2–28
μg/mL for mebeverine hydrochloride and 1–12 μg/mL for chlordiazepoxide. The obtained resultswere statistically
compared with those of the official methods using Student t-test, F-test, and one way ANOVA, showing no
significant difference with respect to accuracy and precision.
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